Zoology

A whole zoo of methods for zoology

Zoology is the study of animals and comprises developmental biology, genetics, biochemistry, and physiology. Various experimental methods are used for zoology research, most frequently light microscopy. In zoology, imaging typically spans several length scales: imaging often starts at the whole organism, continues with tissues or organs, then individual cells are analyzed, and at the final level of resolution, intracellular features such as protein assemblies or organelles are observed. Obstacles in zoological imaging are often intransparent structures or autofluorescent background, which stems from intransparent cells or the cuticle.

Abberior offers several unique microscope features which facilitate imaging of zoological samples at several levels of resolution:

• MINFLUX imaging mode for resolutions down to 2 nm

• MINFLUX single particle tracking with up to 10 kHz, i.e., 1 data point every 100 µs

• Adaptive Illumination for super-resolution STED imaging at minimal light dosages

• Lifetime imaging and advanced time-gating

• Pulsed high-power STED lasers for best resolutions with 2D and 3D super-resolution nanoscopy

• Detectors with superior detection efficiency (up to 65%)

Year	Journal	Author	Title
2011	Biophysical Journal	Mike Friedrich et al.	STED-SPIM: Stimulated Emission Depletion Improves Sheet Illumination Microscopy Resolution
2012	Microscopy & Microanalysis	Caixia Lv et al.	High-Resolution Optical Imaging of Zebrafish Larval Ribbon Synapse Protein RIBEYE, RIM2, and CaV 1.4 by Stimulation Emission Depletion Microscopy
2017	PNAS	Limor Freifeld et al.	Expansion microscopy of zebrafish for neuroscience and developmental biology studies
2019	Journal of Physics	Weichun Zhang et al.	Super‐resolution imaging of densely packed DNA innuclei of zebrafish embryos using stimulated emission double depletion (STEDD) microscopy
2020	Optics Letters	Arik Girsault and Amit Meller	Sub-second, super-resolved imaging of biological systems using parallel EO-STED