Neurobiology

Resolve the mind

Research in neuroscience is centered around the structure and function of neurons and the nervous system. It combines disciplines like (electro-)physiology, anatomy, molecular, and developmental biology, to name a few.

Often, neuroscience hinges on imaging cultured neuronal cells and fixed and alive tissue sections. Light microscopy is a valuable tool here since it analyzes the structure, function, and interaction of neuronal cells and systems in an intact and even living state. Because the size of vesicles, synapses, spines, and other neuronal structures is frequently below the resolution limit of conventional light microscopy, super-resolution methods such as STED and MINFLUX nanoscopy are frequently used for neuroscience research.

Abberior offers several unique microscope features which facilitate imaging of fixed and living neurobiological samples over extended time periods at the highest possible resolution:

• MINFLUX imaging mode for resolutions down to 2 nm

• MINFLUX single particle tracking with up to 10 kHz, i.e., 1 data point every 100 µs

• Adaptive Illumination for super-resolution STED imaging at minimal light dosages

• Lifetime imaging and advanced time-gating

• Pulsed high-power STED lasers for best resolutions with 2D and 3D super-resolution imaging

• Detectors with superior detection efficiency (up to 65%)

Year	Journal	Author	Title
2011	Science	Karen S. Y. Liu et al.	​RIM-Binding Protein, a Central Part of the Active Zone, Is Essential for Neurotransmitter Release
2012	Science	S. W. Hell et al.	Nanoscopy in a Living Mouse Brain
2013	Journal of Neurochemistry	Francisco J Barrantes et al.	STED microscopy of living cells-new frontiers in membrane and neurobiology
2013	​Biophysical Journal	Loew, L. M. et al.	Superresolving Dendritic Spines​
2014	ScienceDirect	Francisco J Barrantes et al.	Recent applications of Super-Resolution microscopy in neurobiology​
2016	​Scientific Reports	S. W. Hell et al.	Subcortical cytoskeleton periodicity throughout the nervous system