Virology

Spotting the enemy with advanced light microscopy

Virology studies the function, genetics, and structure of viruses. In addition, components of viroids, infection, and maturation of virus particles within their host cells are important topics in virology. In the past, virus particles could only be imaged using electron microscopy due to their small size. Today, super-resolution approaches such as STED and MINFLUX nanoscopy facilitate virology research in living and fixed systems.

Abberior offers several unique microscope features which facilitate imaging of virus particles at the highest possible resolution:

• MINFLUX imaging mode for resolutions down to 2 nm

• MINFLUX single particle tracking with up to 10 kHz, i.e., 1 data point every 100 µs

• Adaptive Illumination for super-resolution STED imaging at minimal light dosages

• Lifetime imaging and advanced time-gating

• Pulsed high-power STED lasers for best resolutions with 2D and 3D super-resolution imaging

• Detectors with superior detection efficiency (up to 65%)

Year	Journal	Author	Title
2012	Science	Jakub Chojnacki et al.	Maturation-Dependent HIV-1 Surface Protein Redistribution Revealed by Fluorescence Nanoscopy
2015	Nature Communications	Laine, R., et al	Structural analysis of herpes simplex virus by optical super-resolution imaging
2016	ACS Nano	Hanne, J., et al.	Stimulated Emission Depletion Nanoscopy Reveals Time- Course of Human Immunodeficiency Virus Proteolytic Maturation
2017	PLoS ONE	F. Baharom et al.	Visualization of early influenza A virus trafficking in human dendritic cells using STED microscopy
2017	Bio-protocol	M. Mehedi et al.	Multicolor Stimulated Emission Depletion (STED) Microscopy to Generate High-resolution Images of Respiratory Syncytial Virus Particles and Infected Cells
2019	Methods Mol Biol.	Gray, Robert & Albrecht, David.	Super-resolution Microscopy of Vaccinia Virus Particles.