Plant Sciences

Planting success

Modern plant sciences comprise breeding research, (plant-) developmental biology, genetics, biochemistry, and (plant-) physiology. Various experimental methods are used in plant research, and light microscopy is prominent among them. In plant science, imaging typically spans several length scales: imaging frequently starts at the scale of a whole organism, continues with tissue or sections, then individual cells are analyzed, and at the final level of resolution, intracellular features such as protein assemblies or organelles are observed.

Obstacles in plant imaging are often the sizeable autofluorescent background, which stems from the cuticle or chloroplasts.

Abberior offers several unique microscope features which facilitate imaging of plant samples at several levels of resolution:

• MINFLUX imaging mode for localization precisions down to 2 nm

• MINFLUX single particle tracking with up to 10 kHz, i.e., 1 data point every 100 µs

• Adaptive Illumination for confocal and super-resolution STED nanoscopy at a minimal light dose

• Advance time gating for confocal and super-resolution STED nanoscopy at a minimal light dose

• Advance time gating for confocal and super-resolution STED nanoscopy for reducing autofluorescent background

• Pulsed high-power STED lasers for best resolution in 2D and 3D super-resolution imaging

• Detectors with superior detection efficiency (up to 65%)

Year	Journal	Author	Title
2015	Bioessays	Eugenio F. et al.	Super-resolution imaging for cell biologists
2017	Frontiers in Plant Science	Veit Schubert	Super-resolution Microscopy Applications in Plant Cell Research