Physiology

Physiology − adaptation is key

Physiology is centered around the analysis of the structure and function of cells and tissues. It combines disciplines such as (electro-)physiology, anatomy, and molecular biology.

Vastly different length scales have to be taken into account: from the whole organism, over tissues or organs, then individual cells, and – at the final level of resolution – intracellular features such as protein assemblies or organelles.

Light microscopy is one of the most important techniques for analyzing cells' and systems' structure, function, and interaction. Because the size of (sub-) cellular structures is often below the resolution limit of conventional light microscopy, super-resolution approaches like STED and MINFLUX nanoscopy are frequently used for physiology research.

Abberior offers several unique microscope features which facilitate imaging of fixed and living samples over extended time periods at the highest possible resolution:

• Adaptive Optics for super-resolution tissue imaging at vast depths

• MINFLUX imaging mode for resolutions down to 2 nm

• MINFLUX single particle tracking with up to 10 kHz, i.e., 1 data point every 100 µs

• Adaptive Illumination for super-resolution STED imaging at minimal light dosages

Year	Journal	Author	Title
2012	Cold Spring Harbor Protocal	Katrin I. et al.	Stimulated Emission Depletion (STED) Imaging of Dendritic Spines in Living Hippocampal Slices
2013	Biophysical Journal	Kevin T. et al.	Live-Cell Superresolution Imaging by Pulsed STED Two-Photon Excitation Microscopy
2013	The Journal of Neuroscience	Deepak Nair et al.	Super-Resolution Imaging Reveals That AMPA Receptors Inside Synapses Are Dynamically Organized in Nanodomains Regulated by PSD95
2013	Biophysical Journal	Philipp Bethge et al.	Two-Photon Excitation STED Microscopy in Two Colors in Acute Brain Slices
2014	PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY	Aude Panatier et al.	Dissecting tripartite synapses with STED microscopy