Microbiology

Nothing is too small to be resolved

Microbiology comprises areas such as bacteriology and mycology. During the foundation of this field, microorganisms like bacteria and fungi were characterized using transmitted light microscopy, whereas today, most studies use fluorescence light microscopy. Microbiologists investigate gene expression, host-pathogen interactions, the live cycle of organisms, infection biology, bacterial cell biology, and many more.

The size of many microorganisms is in the range of the resolution limit of conventional light microscopy. Thus, the analysis of substructures often is not possible without the resolving power of STED and MINFLUX nanoscopy.

Abberior offers several unique microscope features which facilitate imaging of fixed and living neurobiological samples over extended time periods at the highest possible resolution:

• MINFLUX imaging mode for resolutions down to 2 nm

• MINFLUX single particle tracking with up to 10 kHz, i.e., 1 data point every 100 µs

• Adaptive Illumination for super-resolution STED imaging at minimal light dosages

• Lifetime imaging and advanced time-gating

• Pulsed high-power STED lasers for best resolutions in 2D and 3D super-resolution nanoscopy

• Detectors with superior detection efficiency (up to 65%)

Year	Journal	Author	Title
2017	Methods	N.K. Sarangi et al.	Unraveling complex nanoscale lipid dynamics in simple model biomembranes: Insights from fluorescence correlation spectroscopy in super-resolution stimulated emission depletion mode
2018	NATURE COMMUNICATIONS	Kamper M et al.	Near-infrared STED nanoscopy with an engineered bacterial phytochrome
2019	International Journal of Molecular Sciences	Cramer K et al.	Visualization of Bacterial Protein Complexes Labeled with Fluorescent Proteins and Nanobody Binders for STED Microscopy