STED 活細胞成像應用

了解STED技術如何應用在活細胞成像領域

光學顯微鏡可用於分析未受干擾的活細胞和組織內的動力學和結構。這是光學顯微鏡區別於其他類型顯微鏡的關鍵。

對於活細胞成像，其水浸物鏡、自動聚焦裝置和環境控制等一系列元件都非常關鍵，但長時間活細胞成像的關鍵在於最大限度地減少照射到樣品上的光劑量。

Abberior 提供多種獨特的硬體和軟體功能，以幫助活細胞和組織成像。

Year	Journal	Author	Title
2008	PNAS	Hein, B. et al.	Stimulated emission depletion (STED) nanoscopy of a fluorescent protein-labeled organelle inside a living cell
2010	Biophys. J.	Hein, B. et al.	Stimulated Emission Depletion Nanoscopy of Living Cells Using SNAP-Tag Fusion Proteins
2011	Biophys. J.	Rankin, B. R. et al.	Nanoscopy in a Living Multicellular Organism Expressing GFP
2013	Nature Chem.	Lukinavicius, G. et al.	A near-infra-red fluorophore for live-cell super-resolution microscopy of cellular proteins
2014	Nature Meth.	Lukinavicius, G. et al.	Fluorogenic probes for live-cell imaging of the cytoskeleton
2015	Nature Commun.	Lukinavicius, G. et al.	SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy
2016	Nat Commun.	Bottanelli F. et al.	​Two-colour live-cell nanoscale imaging of intracellular targets.
2016	Angew Chem Int Ed	Butkevich, A. N. et al.	Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super-Resolution STED Microscopy in Living Cells